ZetaSizer Ultra - Malvern (ZETASIZER)

Instrument status:
Operational

Equipment placement:
CEITEC Nano - B1.21

The ZetaSizer Ultra from the Malvern company provides the ability to measure the size and zeta potential of particles, molecules or micelles in a liquid medium. The unique features of the ZetaSizer for measurements over a wide concentration range and precise temperature control give reproducible, repeatable and accurate results. This device utilizes Multi-Angle Dynamic Light Scattering (MADLS®) technology for the highest resolution of sizing data.

The ZetaSizer works in the sample temperature range of 0–120 °C. The minimum sample volume is 3 μL for the particle size and 20 μL for the zeta potential measurement. The minimum sample concentration for particle size measurement depends on the technique used, starting from 0.1 mg/mL for Dynamic light scattering (Non-Invasive Back-Scatter) to 1 mg/mL for MALDS, the same concentration as for zeta potential. The size range is 0.3–10 μm for size determination and 3.8 nm–100 um for zeta potential measurement.

Publications:

TINOCO NAVARRO, L.; CIHLÁŘ, J.; MICHALIČKA, J.; KAŠTYL, J.; ČÁSTKOVÁ, K., 2023: Effect of MCAA Synthesis and Calcination Temperature on Heterojunction Formation and Photocatalytic Activity of Biphasic TiO2 (B/A). CATALYSIS LETTERS 2, doi: 10.1007/s10562-023-04489-7; FULL TEXT
(RIGAKU3, WITEC-RAMAN, FTIR, VERIOS, TITAN, KRATOS-XPS, BET-DEGASSER, BET-ANAMET, ZETASIZER)

Photogallery

Specification

A) Particle size and molecular size

Measurement principle	Non-Invasive Back Scatter (NIBS) Dynamic Light Scattering Dynamic Light Scattering (90° and 13°) Multi-Angle Dynamic Light Scattering (MADLS)
Measurement angle	173°, 13°, 90°
Measurement range	Diameter: 0.3 nm – 15 μm ^{(2) (9)}
Minimum sample volume	3 μL
Concentration range	Minimum sample concentration: Blue Label: 0.2 mg/mL 15 kDa Protein Red Label: 0.1 mg/mL 15 kDa Protein MADLS: 0.1 mg/ml 15 kDa Protein MADLS Particle Concentration Enabled? Blue Label: No Red Label: Yes Maximum sample concentration ⁽³⁾: 40% w/v

B) Zeta potential

Measurement principle	Mixed-Mode Measurement phase analysis light scattering (M3-PALS)
Size range	3.8 nm – 100 μm
Minimum sample volume	20 μL ⁽⁴⁾
Concentration range	Red Label: 1 mg/mL (5) to 40% w/v ⁽⁶⁾ Blue Label: 10 mg/mL (5) to 40% w/v ⁽⁶⁾
Sample conductivity range	Maximum: 260 mS/cm

C) Static Light Scattering (molecular weight)

Molecular weight range	300 Da – 20 mDa
Measurement principle	Static Light Scattering with Debye Plot
Accuracy	+/- 10% typical

D) Particle concentration

Measurement principle	Blue Label: N/A Red Label: MADLS - Particle Concentration (Mie)
Concentration range	Blue Label: N/A Red Label: 1x108 to 1x1012 particles/mL
Calibration type	Blue Label: N/A Red Label: Single point system calibration only

Measurement principle

Blue Label: N/A

Red Label: MADLS - Particle Concentration (Mie)

Concentration range

Blue Label: N/A

Red Label: 1x108 to 1x1012 particles/mL

Calibration type

Blue Label: N/A

Red Label: Single point system calibration only

E) System

Detector	Avalanche Photodiode
Laser safety	He-Ne (633 nm) Blue label: 4 mW Max Red label: 10 mW Max
Temperature control range	0°C to 120°C (7)
Ambient conditions	+10°C to +35°C, 35 - 80% RH (non-condensing)

F) Notes

Notes	⁽¹⁾ Water as dispersant ⁽²⁾ Indicative data available for particle sizes greater than 10 μm when using Extended Size Range analysis and low volume disposable sizing cell accessory. ⁽³⁾ Sample dependent ⁽⁴⁾ Using patented diffusion barrier technique ⁽⁵⁾ Measured using 15 kDa protein ⁽⁶⁾ Measured using bile acid ⁽⁷⁾ Dry nitrogen cell purge required for temperatures below dewpoint ⁽⁸⁾ Accuracy 0.1°C at 25°C, 0.2°C at 0°C, 0.5°C at 90°C ⁽⁹⁾ Recommended upper size range for MADLS is 500nm diameter

Notes

⁽¹⁾ Water as dispersant
⁽²⁾ Indicative data available for particle sizes greater than 10 μm when using Extended Size Range analysis and low volume disposable sizing cell accessory.
⁽³⁾ Sample dependent
⁽⁴⁾ Using patented diffusion barrier technique
⁽⁵⁾ Measured using 15 kDa protein
⁽⁶⁾ Measured using bile acid
⁽⁷⁾ Dry nitrogen cell purge required for temperatures below dewpoint
⁽⁸⁾ Accuracy 0.1°C at 25°C, 0.2°C at 0°C, 0.5°C at 90°C
⁽⁹⁾ Recommended upper size range for MADLS is 500nm diameter

Documents

Here is place for your documents.

ZetaSizer Ultra - Malvern (ZETASIZER)

Publications:

Photogallery

Specification

A) Particle size and molecular size

B) Zeta potential

C) Static Light Scattering (molecular weight)

D) Particle concentration

E) System

F) Notes

Documents

Research

Services

People

Education

Jobs

About Us

You can find us in the heart of Europe