CEITEC Nano Research Infrastructure
Brno University of Technology
Purkyňova 123, 612 00 Brno
Czech Republic
+420 54114 9207
nano@ceitec.vutbr.cz
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ZetaSizer Ultra - Malvern (ZETASIZER)
Guarantor:
Michela Sanna, Ph.D.
Instrument status:
Operational
Equipment placement:
CEITEC Nano - B1.15
Upcoming trainings:
17.2. 09:30 - 12:30:
DLS-ZetaSizer Part 1+2 -
This training will provide hands-on practice with basic methods for measuring particle size using the DLS technique.
The session will cover:
-An overview of DLS principles, including light scattering and Brownian motion.
-Step-by-step guidance on preparing samples for size and zeta potential measurements.
-The use of different measurement cells (e.g., disposable and reusable cuvettes) and their applications for specific types of samples.
Practical demonstration of data acquisition, optimization of measurement parameters, and analysis using the Malvern software interface.
By the end of the training, participants will gain both theoretical insights and practical expertise in using the Malvern device for comprehensive particle characterization.
The ZetaSizer Ultra from the Malvern company provides the ability to measure the size and zeta potential of particles, molecules or micelles in a liquid medium. The unique features of the ZetaSizer for measurements over a wide concentration range and precise temperature control give reproducible, repeatable and accurate results. This device utilizes Multi-Angle Dynamic Light Scattering (MADLS®) technology for the highest resolution of sizing data.
The ZetaSizer works in the sample temperature range of 0–120 °C. The minimum sample volume is 3 μL for the particle size and 20 μL for the zeta potential measurement. The minimum sample concentration for particle size measurement depends on the technique used, starting from 0.1 mg/mL for Dynamic light scattering (Non-Invasive Back-Scatter) to 1 mg/mL for MALDS, the same concentration as for zeta potential. The size range is 0.3–10 μm for size determination and 3.8 nm–100 um for zeta potential measurement.
Publications:
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POLÁKOVÁ, V.; MATULOVÁ, J.; BRTNÍKOVÁ, J.; FOHLEROVÁ, Z.; ŠMERKOVÁ, K.; KAISER, J.; ZIKMUND, T.; PROCHÁZKOVÁ, P.; ŽÍDEK, J.; VOJTOVÁ, L., 2025: In Situ Homogeneous Generation of Copper Nanoparticles in Collagen-Cellulose Freeze-Dried Foams Using Natural Reduction Agents to Enhance Their Stability, Antibacterial Properties, and Cytocompatibility. ACS OMEGA 10(35), p. 39799 - 39813, doi: 10.1021/acsomega.5c03661; FULL TEXT
(MIRA-STAN, ZETASIZER, LEICACOAT-STAN, micro-CT-L240) -
Hashemi, A.; Ezati, M.; Zumberg, I.; Chmelíková, L.; Fohlerová, Z.; Provazník, V., 2025: In vitro evaluation of 3D-printed conductive chitosan – polyaniline scaffolds with exosome release for enhanced angiogenesis and cardiomyocyte protection. RSC ADVANCES 15, p. 16826 - 16844, doi: 10.1039/d5ra02940f; FULL TEXT
(TITAN, FTIR, ZETASIZER) -
TINOCO NAVARRO, L.; CIHLÁŘ, J.; MICHALIČKA, J.; KAŠTYL, J.; ČÁSTKOVÁ, K., 2023: Effect of MCAA Synthesis and Calcination Temperature on Heterojunction Formation and Photocatalytic Activity of Biphasic TiO2 (B/A). CATALYSIS LETTERS 2, doi: 10.1007/s10562-023-04489-7; FULL TEXT
(RIGAKU3, WITEC-RAMAN, FTIR, VERIOS, TITAN, KRATOS-XPS, BET-DEGASSER, BET-ANAMET, ZETASIZER) -
VISHAKHA, V.; ABDELLATIF, A.; MICHALIČKA, J.; WHITE, P.; LEPCIO, P.; TINOCO NAVARRO, L.; JANČÁŘ, J., 2023: Carboxymethyl starch as a reducing and capping agent in the hydrothermal synthesis of selenium nanostructures for use with three-dimensional-printed hydrogel carriers. ROYAL SOCIETY OPEN SCIENCE 10(10), p. 1 - 17, doi: 10.1098/rsos.230829; FULL TEXT
(ZETASIZER, RIGAKU3, VERIOS, MIRA-STAN, LEICACOAT-STAN, TITAN, FTIR-CHEMLAB, KRATOS-XPS, JASCO) -
KADLECOVÁ, Z.; SEVRIUGINA, V.; LYSÁKOVÁ, K.; RYCHETSKÝ, M.; CHAMRADOVÁ, I.; VOJTOVÁ, L., 2023: Liposomes Affect Protein Release and Stability of ITA-Modified PLGA-PEG-PLGA Hydrogel Carriers for Controlled Drug Delivery. BIOMACROMOLECULES 25(1), p. 67 - 10, doi: 10.1021/acs.biomac.3c00736; FULL TEXT
(DAWN-HELEOS, ZETASIZER, NMR, MIRA-STAN)
Photogallery
Specification
A) Particle size and molecular size
| Measurement principle | Non-Invasive Back Scatter (NIBS) Dynamic Light Scattering |
|---|---|
| Measurement angle | 173°, 13°, 90° |
| Measurement range | Diameter: 0.3 nm – 15 μm (2) (9) |
| Minimum sample volume | 3 μL |
| Concentration range | Minimum sample concentration: Blue Label: 0.2 mg/mL 15 kDa Protein Red Label: 0.1 mg/mL 15 kDa Protein MADLS: 0.1 mg/ml 15 kDa Protein MADLS Particle Concentration Enabled? Blue Label: No Red Label: Yes Maximum sample concentration (3): 40% w/v |
B) Zeta potential
| Measurement principle | Mixed-Mode Measurement phase analysis light scattering (M3-PALS) |
|---|---|
| Size range | 3.8 nm – 100 μm |
| Minimum sample volume | 20 μL (4) |
| Concentration range | Red Label: 1 mg/mL (5) to 40% w/v (6) Blue Label: 10 mg/mL (5) to 40% w/v (6) |
| Sample conductivity range | Maximum: 260 mS/cm |
C) Static Light Scattering (molecular weight)
| Molecular weight range | 300 Da – 20 mDa |
|---|---|
| Measurement principle | Static Light Scattering with Debye Plot |
| Accuracy | +/- 10% typical |
D) Particle concentration
| Measurement principle | Blue Label: N/A Red Label: MADLS - Particle Concentration (Mie) |
|---|---|
| Concentration range | Blue Label: N/A Red Label: 1x108 to 1x1012 particles/mL |
| Calibration type | Blue Label: N/A Red Label: Single point system calibration only |
E) System
| Detector | Avalanche Photodiode |
|---|---|
| Laser safety | He-Ne (633 nm) |
| Temperature control range | 0°C to 120°C (7) |
| Ambient conditions | +10°C to +35°C, 35 - 80% RH (non-condensing) |
F) Notes
| Notes | (1) Water as dispersant |
|---|
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