Confocal Laser Scanning Microscope Olympus Lext OLS4100 (LEXT-OLS4100)

Confocal Laser Scanning Microscope Olympus Lext OLS4100

Petr Lepcio, Ph.D.

Instrument status:
Operational Operational, 9.3.2020 17:10, recently moved to Optical Lab - C1.21 (Nanocharacterization)

Equipment placement:
CEITEC Nano - C1.21

Confocal Laser Scanning Microscopy (CLSM) is a combined optical imaging microscope which utilizes a set of optical lenses and a point scanning by monochromatic laser beam. 2D and 3D image of surface is acquired thanks to the point-scanning and confocal principle (single-plane acquisition). CLSM expands the possibilities of simple optical microscopes while it does not require prior surface preparation and is not as limited in terms of scanning range as scanning electron microscopy (SEM). It is capable to measure polymers, composites, ceramics, wood, bones, teeth, etc. including materials degraded by various means such as microorganisms, UV radiation, mechanical loading, etc. There are two sets of objectives with both short and long (rough surfaces) working distance mounted on a motorized software-controlled revolver, samples are placed on motorized software-controlled stage



A confocal laser scanning microscope for 2D and 3D optical observation.


Optical microscopy in full-color spectrum

Confocal laser scanning microscopy (CLSM)

  • 3D image of surface
  • Contactless roughness measurement (line, area)

Exchangeable revolver heads with lenses

Short (SWD) and Long (LWD) Working Distance Lenses

Motorized stage with automatic stitching feature

Lext software with tools for profile, roughness (Line, Surface), geometry, area/volume and particle analysis

Confocal Laser Scanning Microscope (CLSM)

Olympus Lext OLS4100

Light Source (CLSM)
405 nm Semiconductor Laser
Detector (CLSM)
Light Source (Optical Mode)
White LED
Detector (Optical Mode)
1/1.8“ 2 MPx Single-Panel CCD
Planar resolution
0.12 μm
Planar measurement – accuracy
± 2 %
Height measurement – Movement resolution
10 nm
XY-Motorized stage
(100 × 100) mm
Revolver head 1: 2.5× (LWD), 5× (LWD), 10× (LWD), 20× (SWD), 50× (SWD), 100× (SWD)

Revolver head 2: 10× (LWD), 20× (LWD), 50× (LWD), 100× (LWD)


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